Mitochonderial Cytochrome C Oxidase Subunit 1 ( cox 1 ) Gene Sequence of the Hymenolepis Species

In the current study, Mitochondrial Cytochrome c oxidase gene especially codons within subunit 1 (cox1) of H. diminuta and H. nana Egyptian isolates from two stages (adult worms and eggs) and hosts origin (human and rat) were amplified, sequenced and aligned. PCR products were approximately 700 bp, 702 bp and 715 bp of H. nana rat isolates, H. diminuta rat isolates, and H. nana human isolates, respectively. Moreover, despite their host susceptibility differences they all gathered in one cluster with three genbank published isolates of H. nana; AB033412.1, AB494472.1 and AY121842.1), forming one clade with 100% similarity, which was non significantly decreased on internal nodes. In addition, clearly far away from H. diminuta published sequence AB033412.1 who's assumed to be genetically closely related to Egyptian H. diminuta than all other H. nana isolates. Both Egyptian murine isolates of Hymenolipidid; H. diminuta and H. nana, were closer to each other than being to H. nana of human origin. The annotated sequences of Egyptian isolates were deposited in GenBank under the following accession numbers; H. diminuta (GU433102), H. nana rat isolate (GU433103), and H. nana human isolate (GU433104). Finally, the development of effective control strategies will only be possible if complete understanding of the epidemiology of infection is elucidated. [Omnia M. Kandil, Mona S. Mahmoud, Nesreen A.T. Allam, Amira H. El Namaky. Mitochonderial Cytochrome C Oxidase Subunit 1 (cox 1) Gene Sequence of the Hymenolepis Species. Journal of American Science 2010;6(12):640-647]. (ISSN: 1545-1003). http://www.americanscience.org.